منابع مشابه
STED nanoscopy with mass-produced laser diodes.
We show that far-field fluorescence nanoscopy by stimulated emission depletion (STED) can be realized with compact off-the-shelf laser diodes, such as those used in laser pointers and DVDs. A spatial resolution of 40-50 nm is attained by pulsing a 660 nm DVD-diode. The efficacy of these low-cost STED microscopes in biological imaging is demonstrated by differentiating between clusters of the sy...
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We demonstrate stimulated emission depletion (STED) microscopy with 20 nm gold nanospheres coated by fluorescently doped silica. We demonstrate significantly improved spatial resolution down to 75 nm, which is the first time that hybrid NPs are used in STED imaging beyond the diffraction limit of confocal microscopy. Unlike previous demonstrations of super-resolution with metal nanoparticles wi...
متن کاملSTED nanoscopy with fluorescent quantum dots
The widely popular class of quantum-dot molecular labels could so far not be utilized as standard fluorescent probes in STED (stimulated emission depletion) nanoscopy. This is because broad quantum-dot excitation spectra extend deeply into the spectral bands used for STED, thus compromising the transient fluorescence silencing required for attaining super-resolution. Here we report the discover...
متن کاملDual-label STED nanoscopy of living cells using photochromism.
We demonstrate live-cell STED microscopy of two protein species using photochromic green fluorescent proteins as markers. The reversible photoswitching of two markers is implemented so that they can be discerned with a single excitation and STED wavelength and a single detection channel. Dual-label STED microscopy is shown in living mammalian cells.
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Superresolution microscopy is currently revolutionizing optical imaging. A key factor for getting images of highest quality is - besides a well-performing imaging system - the labeling of the sample. We compared the fluorescent dyes Abberior Star 488, Alexa 488, Chromeo 488 and Oregon Green 488 for use in continuous wave (cw-)STED microscopy in aqueous buffer and in a durable polymer matrix. To...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2017
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.1708304114